This study aimed to determine the requirements and constraints of the application of knowledge management at Tishreen University. The study area was a set of employees in the central administration of Tishreen University. The researcher used the questionnaire as a tool to collect data, where 160 questionnaires were recovered from a total of 200 distributed. The result of this study found an urgent requirement for the application of knowledge management is the organizational culture, organizational structure and information technology, in addition to a number of direct obstacles, including: lack of clarity of the concept of knowledge management at most of the studied sample, and failure to allocate sufficient funding to be applied at Tishreen University.