This research was conducted during the growing season of 2015 at AL-Arema village of governorate Tartus, to study the effect of three spray bio-fertilizer times (1- flowering stage. 2- pods formation stage . 3- seeds formation stage), and four rates of biofertilizer (zero, 2 cm3/liter ,4 cm3/liter,6 cm3/liter) in the growth and productivity of peanut crop. The experiment was designed by using Split-plot design three replications.