DETECTION OF miRNA USING SURFACE PLASMON RESONANCE (SPR)

MicroRNAs (miRNAs) are single-stranded, non-coding RNA molecules, which can regulate the translation of target proteins and thereby control biological functions. They qualify as diagnostic markers and can help to detect diseases earlier. In case of a disease the concentrations of specific miRNAs which are characteristic for it do vary in quantity. For the detection of miRNA we are using a surface plasmon resonance (SPR) biosensor, which allows to measure interactions of molecules at an interface in real-time. For this, thiolated LNA capture probes are immobilized on the gold chip.