This study was conducted on domestic pigeons populations in the provinces of Hama, Idlib and Latakia using several conventional diagnostic techniques , including pathological examination tests and agar gel immune diffusion test and isolation on chicken embryo. The number of suspected birds to be infected by pigeon pox through clinical symptoms and macroscopic lesions were about 37 birds. we noticed the presence of lesions in warts and scars on the nonfeather parts of the face and on the corner of the mouth and eyelids and other areas of the body. the most of these accompanied by the presence of the of defteric lesions on the mucous membrane of the oral cavity. The results showed that all birds suffered from the presence of infection fowlpox through histological examination of skin and difteric lesions. the results has been confirmation by agar gel immune diffusion test. And we successfully isolated the virus that caused the disease by injection on the Chorioallantoic membrane of a chicken's egg fertilized SAN.