The volatile oil of Anthemis wettsteiniana was studied in environmentally different regions distributed on five locations in the provinces of Homs and Hama, in order to determine the quantity and quality and effect of the surrounding environment, on the volatile oil specifications. The plants were gathered during tow phonological phases of the life of the plant included the vegetative growth stage- the full of flowering stage. It was then extracted through water extraction method. It was found that the highest percentage of volatile oil was recorded in Al-afif location at the full of the flowering stage during the 2013 season, where the percentage of the volatile oil was 0.12 (ml/100g) ,while the lowest percentage of volatile oil was recorded in Tal-snan during the 2014 season , the percentage of the volatile oil was 0.04 (ml/100g).