This study was conducted in all health centers that provide vaccine services of Latakia city, on a sample of all the nurses who provide the vaccination for children in health centers , their number (27) nurse, and all the parents of children auditors health center to receive the vaccine their number (270) family. Data collected from October 2013 to March 2014, according to note form to evaluatethe performanceofnursesat administrating vaccines , and aquestionnaire to assess information of nurses, and a questionnaire to assess parents' satisfaction with the nurses' performance.This study aimed to assess the quality of nurses' performance in conducting of vaccination at health centers in Latakiacity.The most important results of this study that the quality of performance for most nurses was average in conducting vaccination. The study also showed that the majority of nurses have a weak level of information about vaccines. but the parents were deeply dissatisfied with nurses' performance in conducting vaccination.This study was conducted in all health centers that provide vaccine services of Latakia city, on a sample of all the nurses who provide the vaccination for children in health centers , their number (27) nurse, and all the parents of children auditors health center to receive the vaccine their number (270) family. Data collected from October 2013 to March 2014, according to note form to evaluatethe performanceofnursesat administrating vaccines , and aquestionnaire to assess information of nurses, and a questionnaire to assess parents' satisfaction with the nurses' performance.This study aimed to assess the quality of nurses' performance in conducting of vaccination at health centers in Latakiacity.The most important results of this study that the quality of performance for most nurses was average in conducting vaccination. The study also showed that the majority of nurses have a weak level of information about vaccines. but the parents were deeply dissatisfied with nurses' performance in conducting vaccination.