Some factors which affect in vitro propagation of sour orange (Citrus aurantium. L.) were studied on Murashige and Skoog medium. Medium containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot proliferation and it enhanced callus formation.