Studying the Presence and Expression of P27 Gene in Promastigotes of Leishmania tropica
published by Aِl-Baath University
in 2017
in Pharmacy
and research's language is
العربية
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Abstract in English
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers between species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
References used
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Dey, R., et al. (2010). "Characterization of a Leishmania stage-specific mitochondrial membrane protein that enhances the activity of cytochrome c oxidase and its role in virulence." Mol Microbiol 77(2): 399-414