This study was conducted on domestic pigeons and back yard chickens populations in many regions of Syrian Arab Republic using techniques of isolation on chicken embryo and cell culture. The birds were suspected to be infected by pox virus through clinical symptoms by the presence of lesions in warts and scars on Different areas of the face as the comb, wattle, the corner of the mouth and eyelids and other areas of body and we notice presence of Diphtheric lesions on the mucous membrane of the oral cavity in many cases.