A successful and detailed in vitro propagation system for rapid micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has been developed. Shoot tips and axillary buds excised from field-grown trees were used as explants, which were surface-disinfected with mercury bichloride, or with solution of sodium or calcium hypochlorite followed by three rinses with sterile distilled water.