The study was carried out at Tishreen University nursery during the spring of 2015 in order to evaluate the effect of the bio-fertilizer EMI on the growth and production of Sponta Potato Solanum tuberosum. Four treatments were used as follows: control, with water irrigation, spraying and irrigation, spraying. The results showed that the use of bio-fertilizer EMI improved the growth of plants, so the surface foliage, foliage index and plant height were increased significantly comparing with the control. The results also showed that the spraying and irrigation treatment was the best with a tuber average weight of 117.7 g/tube obverse (102.7, 115, 109 g/tuber), and a yield of 5864 kg/d obverse (4151, 5569, 5398 kg/d) for treatment (control, water irrigation, spraying).