This study was carried out to evaluate the effect of isolates of Rhizobium leguminosarum in reducing infection of tomato with Fusarium oxysporum f.sp.lycopersici,the causal agent of tomato wilt. The results demonstrated significant reduced in severity disease of plants were inoculated with Rhizobium leguminosarum, the rate reduced of disease severity was 69.21% and in length of browning was 35.14%. Rhizobium leguminosarum increased of plant height with 21.01%, fresh weight of shoot and root increased with 35.32% and 43.35% respectively,while increased the number of fruit with 47.39% and its weight with 32.48%