The effect of different culture medias (water, MS solid, MS 1/2 solid) on germination and growth of heliotropiun hirsutissimum Grauer. in vitro was studied with different concentrations of gibberellic acid (0.001-0.01-0.1 and 1 mg/l). The results showed that best germination rate (80.5%) and root (20mm) and shoot (35mm) growth were obtained in MS solid medium with 0.1mg/l GA3. Addition of 0.1mg/l GA3 to MS1/2 solid medium improved germination rate (75.44%), root (19mm), and shoot (24mm)growth. When GA3 (0.1 mg/l) was added to water medium, germination rate reached (65.33%), as well as root and shoot growth (12.66mm and20mm) alternatively after 2 weeks of planting. Seedling of MS solid medium were transferred into pots contained torp medium to adapting them with outside environment, and then surviving their growth until maturity after 4 weeks.