Fifty-one female and thirty-three male patients who suffered from UTI and attended Al-Thawra General Hospital at Ibb City in Yemen, were examined for the effects of different antibiotics on E. coli isolated from the mid-stream urine samples. The results showed a variable degree of resistance revealed by E. coli isolates. It ranged between ١٠٠٪ ((against Penicillin G and Ampicillin) and zero resistance ((against Kanamycin, Ciprofloxacin, and Gentamicin). Other tested antibiotics showed resistance ranging between ٤٠ and ٣,٣٤٪.