There is considerable evidence supporting the role of gonadotrophins and progesterone on follicular growth and maturation and functional maintenance of the ovary in the hen. Recently, however, there are ever-expanding list of factors may be involved in establishing follicular growth and ovulation currently including germinal disc region, growth factors, macrophages, plasminogen activator, ornithine decarboxylase, inhibin, activin, follistatin, relaxine, arginin vasotosin, oxytocin, prostaglandines, vasoactive intestinal peptide, and their interactions.