The prevalence of herpes simplex virus type 1 and 2 was determined in the following groups: pregnants, neonates, herpes labial group, kidney transplantation group, patients with herpetic eye infection, cardiac catheterization group, cervical carcinoma group ,patients with suspected herpes genitalis infection , and AIDS patients. EIA method was used to detect antibodies to HSV-1 and HSV-2 in sera of studied persons. The results differ from group to another. The prevalence of HSV-2 antibodies was o% in most groups, except for AIDS group ,cervical carcinoma groupand patients with suspected herpes genitalis group ,in which the prevalence was 4% , 7.84% , 10% respectively.