Alterations of the enamel after topical application of bleaching agents, presenting as major consequences are: ions release, increased superficial roughness, stronger bacterial attachment and hardness alteration.The aims of the study were to evaluate the effect of the bleaching agent (35%hydrogen peroxide) for vital teeth by using light source on the release of ions from the enamel surface. our sample consisted of 30 sound enamel surface for control group,30 sound enamel surface for(35%hydrogen peroxide), and then application of light radiation to activate the bleaching agent. Ion Chromatography were used to measure the ions release .