Effect of Seasonal Changes on the Yield of Local Cow Intact Oocytes for in vitro Culturing


Abstract in English

The aim of this research is to determine the effect of seasonal changes on the yield of morphologically intact oocytes for in vitro maturation and consequently for fertilization and embryo production. Determining the impact of seasonal changes helps us to increase the intact oocytes yield, and better use of in vitro culturing technique of cow oocytes. This leads to: benefit from animals of genetic superiority, maintenance the biodiversity of animal genetic resources by using deep-freezing technique of pre/post in vitro fertilized oocytes taken from cow ovary of animals after slaughter. The results of this research paper show that, the intact oocytes yield for in vitro culturing differs accordingly with year seasons. The lowest percentage of intact cells 29.5 were recorded in the period between May and September, whereas the highest percentage of intact cells 42.6% were recorded in the period between October and March. There was significant difference (P<0.001) between the two periods for intact cells.

References used

Al-Katanani, Y. M., Paula-Lopes F. F. and Hansen P. J. (2002). Effect of season and exposure to heat stress on Oocyte competence in Holstein cows. J. Dairy Sci. 85: 390-396
Asdel, S. A. (1964). Patterns of mammalian reproduction. London: Constable & Co. Ltd, 586 – 588
Badinga, L., Thatcher W. W., Diaz T., Drost M. and Wolfenson, D. (1993). Effect of environmental heat stress on follicular development and steroidogenesis in Lactating Holstein cows. Theriogenology 39:797-810

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