This study was carried out at the Faculty of Agriculture, Damascus University, and ACSAD during the year 2001 – 2002 in order to assess the genetic variability of twenty wheat genotypes for osmotic stress tolerance by using PEG-6000 as a water stress induced tool. This trial aimed to develop a semi-natural rapid and effective screening technique in which the optimum induction as well as lethal levels of osmotic stress are well defined . The results revealed that the treatments –0.4 MPa and –1.5 MPa were the optimum induction and lethal levels of osmotic stress, respectively.