This experiment was conducted at two ecologically different regions, Boka, and Gellien, using 3 lines of X.triticosecale Wittmack (372, C.187, and C.G.2) and 6 cultivars of wheat (5 of them were triticum durum Cham1, Cham3, Cham5, Bohoth5, and Haurani, and one of triticum aestivum Cham6), to assess the changes in water content and dry matter in the grains during the period from anthesis to physiological maturity .The results showed that all genotypes had the same moisture content curves, whereas it had seemed that the two durum wheat cultivars (Cham1 and Bohoth5) exhibited a disturbance in the moisture development curves in the first region, and the same observation was noticed on (Cham1, Cham3, and Cham5) in the second region. However, triticale lines had a higher test weight of 1000 grain compared with wheat cultivars in the two regions, and there was a positive relationship between grain fill duration and the weight of 1000 grain, whereas, there was a depression in the test weight of wheat cultivars in the second region in comparison with the first one, but it is associated with an increase of protein percentage, and this might be attributed to temperature elevation during grain fill stage.