In the present study, we tried to compare the sensitivity and the specificity of the rK39 strips and DAT, to serodiagnose the visceral leishmaniasis disease in some endemic villages in south of Syria, in order to apply the best and the easy test in the epidemiological studies, for serodiagnosis this disease not only in symptomatic patients but also in asymptomatic and suspected cases in order to treat them early and rapidly.