Effect of organic and bio fertilizers (Azotobacter and phosphate solubilizing bacteria) on production of potatoes and some soil properties was studied in Tartous. The experiment consisted of 4 treatments (control soil, soil+manure, soil+ biofertilizer and soil+manure+biofertilizer) each replicated four time, for 2004 and 2005 seasons. Total N, available P, available K, and organic matter in soil were determined. Significant increases were observed in most of the inoculated and organic fertilized treatments compared with the control. However, the treatment (soil+manure+biofertilizer) was the best and showed significant increases in potato yield compared with other treatments especially in the next season. However, the second season was better in yield increase in all treatments than the first seacon.