During the three successive seasons of 2002, 2003, and 2004 six populations, viz.,P1,P2,F1,F2,BC1, and BC2 of two single crosses of cotton (Aleppo40 x Aleppo90) and (Aleppo33/1 x Line118) were established and evaluated to explore heterosis, inbreeding depression, heritability and genetic advance under selection for reproductive branches number, number of bolls per plant, boll weight, and ginning percentage. Significant positive heterotic effects were found for all the studied traits in two crosses except ginning percentage (in the second cross). Significant positive heterosis over mid-parent and higher parent for yield components (number of bolls per plant and boll weight) may lead to a positive heterosis for seed cotton yield/plant.