Isolation and identification of Pseudomonas aeruginosa was screened from 15 samples of Syrian oily-soils to determine the ability to produce Lipase enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during 2007-2008 AD. The results showed that there were grown colonies with color of green or yellow-green surrounded with different diameter of clear zone in three samples and the identification by Mowasat Hospital revealed that it was Pseudomonas aeruginosa In addition, the results showed that the optimum conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of production of lipase with highest activity of 63.3% was three days in submerged culture during the optimum conditions. These results illustrated the important of this isolates to produce and control lipase for industrial production.