A survey was conducted to determine the frequency and mean densities of plant-parasitic nematodes associated with cotton crop (Gossypium hirsutum L.) after the harvesting season 2008 in Syria. Results showed that twelve genera of plant-parasitic nematodes were found associated with cotton crop roots. The distribution of nematode genera varied between the Syrian governorates, Pratylenchus spp., Meloidogyne spp. and Rotylenchulus infect most cotton fields in all syrian governorates. The distribution was relatively uniform between the governorates. They occurred in 80.7%, 46.6% and 32.95%, respectively, of tested fields with an average density of 137.2, 59.6 and 574.3 juveniles/100 cm3 soil, respectively. meanwhile, the genus Tylenchorhynchus was found in central region only. Rotylenchulus was more prevalent in the heavier soils, but Meloidogyne. was not influenced by soil type. The other nematode genera detected in this survey were less frequent, their frequency of occurrence were for Hoplolaimus (19.3%), Scutellonema (31.8%), Tylenchorhynchus (31.8%), Helicotylenchus (30.7%), Rotylenchus (36.4%), Tylenchus (18.2%), Xiphinema (3.4%).