Development of Analytical Method for the Determination of Carbinoxamine Maleate, Dextromethorphan Hydrobromide and Pseudoephedrine Hydrochloride by HPLC


Abstract in English

A simple and accurate method was developed for the analysis of carbinoxamine maleate, dextromethorphan hydrobromide and pseudoephedrine hydrochloride content in pure form and pharmaceutical preparations using HPLC. Analysis was conducted on a silica column (6 μm) with mobile phase consisting of ethanol – ammonium acetate (0.05 M) in rate [85:15] respectively, and at detection wavelength of 276 nmand flow rate 1ml/ min. Results were linear (correlation coefficient R > 0.9996) in the range of the studied concentrations for the active materials. The relative standard deviations (n=6) of intra and interday assay were 0.931%, 1.527% for carbinoxamine maleate and 0.717%, 1.058 % for dextromethorphan hydrobromide and 0.309%, 0.891% for pseudoephedrine hydrochloride, respectively. This method, proved to be easy, precise and economical, is useful for quality control of pharmaceutical drugs industrial samples.

References used

Duterhoff, H. und Kover, K-M. (1977). Identifizierung von Arzneistoffen, Stuttgart, Germany
David Harvey. (2000). Modern Analytical chemistry, Depauw University, USA
Silverstem, R.M. and Webster, F. X. (1998). Spectrometric Identification of Organic Compounds, sixth Edition State of University of New York

Download