Sixty yeasts spp. were isolated from samples of whey which collected from different locations in Syria. These yeasts were classified morphologically according to Barnett’s and physiologically by API technique to identify the species of Yeasts, where had found 10 classified yeasts as follows: Candida krusei is the most of yeasts which founded in whey with 21.66 %, and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came (Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with 11.66%, after that the Candida magnolia is ranked in the fourth rank by distributing with 8.33%. as soon as the Candida famata came in the fifth rank with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%, finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank with 3.33%, so we noticed the variety of the yeasts in the studied locations in Syria.