Aniseed Crop, Pimpinella anisum L. is one of the important medicinal and aromatic plants in Syria and the world. It has very important economic value, appear in its medicinal and multi-uses. Weeds considered as the greatest obstruct for aniseed production and hand picking is commonly used for weed control. So the investigation was carried out to evaluate the effect of the herbicide, Afalon on the weeds of aniseed and its phytotoxicity. Afalon treatment resulted in high efficacy for the broad leaves weeds (92.4 %, 97.1 % and 96.1 %) for the half rate, field rate and double field rate, respectively and (5.8 %, 23.9 % and 29.1 %) for the narrow weeds. The treatment of double field rate gave a high yield (715.3 Kg/ha.) that was equal to weeded plots (777.8 Kg/ha.), and the field rate treatment (673.6 Kg/ha.) was significantly superior to other treatment. This indicated the importance of spraying aniseed crop with Afalon especially when hand picking not possible. Phytotoxicity effect were not recorded on anise plants. Some other plants were not affected by the herbicide like Ammi majus and coriander, those are belong to the same family, Apiaceae.