This study aims to in vivo evaluate the direct microleakage, in class V composite restoration after isolation using the modified metal matrix or gingival retraction cord impregnated with Adrenaline. Materials and Methods: the research sample consisted of 15 patients; each patient has two premolars at least prepared to be extracted for Orthodontic reasons in the same jaw. This sample divided into two groups according to the place, the right premolars group and the left premolars group, whether was upper or lower. Class V cavity was prepared with a specific dimensions on the buccal surface only, then isolated teeth of the first set by modified metal matrix while isolated teeth of the second set by a gingival retraction cord impregnated with Adrenaline. Teeth restored by composite resin, and then was extracted in the same meeting and studied the microleakage winning directly during restoration. .Results: there was statistical difference between the modified metal matrix and the gingival retraction cord impregnated with Adrenaline. Because modified metal matrix had achieved the lowest leakage rate.