Aim of Study: The antimicrobial intracanal medication was used widely, and the most common was calcium hydroxide dressing, which must be removed from the root canal before obturation, hence this study aimed to comparative efficacy of EndoVac and NaviTip 30 G irrigation Needles with Naocl and CHX 2% in removing calcium hydroxide dressing from the apical third of root canal. Materials &Methods: Forty single root and canal teeth were decoronated to the length of 16 mm. Each root was prepared to the apical foramen using ProTaper Universal Hand System to size 40. Each tooth was embedded in silicone putty to create a set matrix, which allowed reassembly of the tooth for irrigation tests after splitting longitudinally. Then each tooth was split into two halves and an artificial groove (L:4, W:0,2 , D:0,5 ) was cut in one half of the root canal wall 1 mm from the apex ,which was then filled with a Ca(OH)2 paste. Digital images were taken before reassembling under stereomicroscope X40. Subsequently the two halves of roots were reassemble with wax , and remounted in their silicone matrixes, then divided into 2 groups.