Using the plant tissue culture techniques in micropropagation and conservation of the endangered Red Doumani grape cultivar
published by Damascus University
in 2012
in
and research's language is
العربية
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Abstract in English
In this study, we used plant tissue culture techniques for micropropagation
of the endangered Red Doumani cultivar. The explants which were the apex
tips and axillary buds (0.5-1 cm) have been cultured on the free of hormones
initial medium WPM (Woody plant medium), then moved to the
micropropagation media. The results showed that the medium containing 4.44
mM BA + 0.58 mM IAA had the best effect on number of new shoots formed
(2.6) and the growth started after 9.22 days, the best elongation was on the
medium supplemented with 4.6 mM KIN + 0.58 mM IAA and the rate of
multiplication reached 5.15 shoots.
References used
ALizadh, M., Singh, S., Patel, V. (2009). Comparative performance of in vitro multiplication in four grape rootstock genotypes. International journal of plant production 4(1): 41-50
Baydar,N.G.(2000). Study of adventitious shoot formation in leaves of grape (Vitis spp). Turkish. Journal. of Biology. 24(3):645-656
Beauchesne, G. (1982). Appearance of plants not true to type during in vitro plant propagation,. In: Variability in plants regenerated from tissue culture. (E.D.): Ealre and Y. Demarly. Praeger publication, New York, 268- 272