A total of 294 samples of raw-unpasteurized bovine milk and variety of dairy products (hard cheese, sweet cheese, cream cheese and cream), were collected from local markets in Lattakia city as well as 44 samples of used water (clear potable water collected before used in cheese preparing processes) and preserving water (turbid water collected from hard cheese preserving tanks). All samples were analyzed for E.coli O157: H7 detection.