Effect of In vitro maturation, fertilization and culture media on bovine embryo production


Abstract in English

This study was conducted at the biotechnology laboratory of AnimalWealth Research Administration, General Commission for Agricultural Research in order to compare the effect of different media on In vitro maturation (oocytes matured at 39 C° under 5% CO2 for 24 h), fertilization، and culture (7 days postfertilization) in humidified atmosphere 95% on bovine embryo production.

References used

Ahuja، K. K. 1985. Carbohydrate determinants involved in mammalian fertilization. Am. J. Anat.، 114:207-225
Atef، A and M. A. Sirard. 2002. Effect of the absence or presence of various protein supplements on further development of bovine oocyte during In vitro maturation. Biol. Reprod.، 66: 901-905
Ax، R. L.، S. M. Bushmeyer، S. K. Boehm and M. E. Bellin. 1984. Binding of the glycosaminoglycan heparin to bovine granulosa cells varies with size and estrogen content of ovarian follicles. Endocr. Res.، 10: 63-72

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