This study was conducted to determine the prevalence of salmonella in the bowels of the broiler carcass in poultry slaughterhouses in southern Syrian governorates. 216 random samples of giblets included liver, spleen, heart and gizzard) collected weekly during March and September of 2011 from small slaughterhouses in southern governorates were incubated in buffered peptone water, selinite broth, Salmonella-Shigella agar and Hikton agar media. The suspected colonies were subjected for staining and microscopically examination. as well as to biochemical tests.