This research was conducted at the Agriculture Faculty, Damascus university to determine some factors affecting rooting of some grape rootstocks (B41, Ru140, SO4) by hardwood cutting during two subsequent seasons. The results showed that Rooting percentage ranged between 50 to 65%. with significant differences between tested rootstocks. The highest rooting percentage was obtained on B41 rootstock reached 65.47% followed by SO4 and then by Ru140 which gave the lowest percentage 50.44%. The treatment with hormone IBA increased rooting proportion and all the parameters (number and length of root, number of leaves and shoot length) were significantly higher than parallel parameters of the control. The storage of cuttings at 4°C for 50 days increased significantly proportion of rooting and number of roots compared with the storage under field condition. There were significant differences among dates of planting where the third date (mid February) showed superiority in all parameters studied over other dates. The cuttings from basal and middle of annual growth gave a significant superiority in the average root length (5.26–4.32 cm, successively) compared to the terminal cuttings while there was no significant effect to the cuttings position on rooting proportion.