This study was conducted at the laboratory of Food Science Department, Agriculture College, Damascus University to isolate and characterize of Lactobacilli bacteria from some Syrian foods and detection the effectiveness of anti-pathogenic bacterial growth. Lactic acid bacteria from different dairy products, pickles and dried plant samples were isolated and identified according to common characteristics. Results showed that twenty-three isolates were rods while only 2 of the them were coccid. These isolates were tested for their antimicrobial effect against pathogenic bacteria for both gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial effect against the three tested bacteria, of which CP50 and CP46 isolates showed the best antimicrobial effect. Results of the standard physiological and biochemical tests identified fourteen isolates as Lactobacillus plantarum and one isolate as Lactobacillus fermentum.