This research was carried out at the farm of Faculty of Agriculture, Damascus University, during 2010-2011 growing season to determine the effect of seedling rate on yield traits (plant number at harvesting, plant height, number of umbels/plant, number and weight of seeds/ umbel, plant dry weight, seed yield and harvest index.) of four ecogenotypes of Anise plants, (Pimpinella anisum L.) collected from different places of Syria: Damascus countryside (Hena, Saasaa), Homs and Edlib. Sowing rates were 10, 15 and 20 Kg/ha.. Trial was laid out using completely randomized block design with three replicates for each treatment. Results showed that studied traits differed according to seeding rate and the ecotype. There were significant differences for the treatment of seeding rate of 15 kg/ha., on seed yield. Regardless the ecotype, increasing the seeding rate up to 20 kg/ha. resulted in maintenance of highest number of plants/unit area, but accompanied with reduction in fertile umbels and seed weight. Statistical analysis showed that the ecotype of Edlib recorded the highest seed weight/ plant and highest seed yield/ planted area, especially with the seeding rate of 15 kg/ha. However, the results showed the seed components of Anise (plant number at harvesting, weight of seeds/ plant) and seed yield were affected by the ecotype and seeding rate.