Factors affecting leaf protoplast isolation from Binella -potato cultivar


Abstract in English

In this research, protoplast isolations were obtained from the leaves of Potato Binella cultivar at the laboratories of Agriculture College- Damascus University in order to determine factors affecting the yield and viability of protoplast isolation. Results indicated that the best yield percentage of isolated protoplast.

References used

Assani, A., R. Haicour, G. Wenzel, W. B. Foroughi, F. Bakry, F.X. Cote, G. Ducreux, A. Ambroise and A. Grapin. 2001. Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci., 162: 355-362
Avihai P., D. Aviv and E. Galun. 1988. Ethylene and in vitro culture of potato: suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.Plant Cell Rep., 7:403- 406
Babaoglu, M. 2000. Protoplast isolation in Lupin (Lupinus mutabilis: sweet) and determination of optimum expplant sources and isolation conditions. Turk. J. Botanical, 24: 177-185

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