The research was carried out in Nawa area in Daraa governorate during the year 2012, to study the response of four sesame genotypes (Local Idleb, Zouri, Improved Hourani and Brown Sudani) to four irrigation treatments (rainfed cultivation, one supplemental irrigation during branches formation, two supplemental irrigations during branche and capsules formation and three supplemental irrigations during branches formation, capsules formation and seed filling stages). The experiment was laid out in split plot design with three replications. The statistical analysis clearly indicated to the existence of genetic variation in (p < 0.05) the response of the studied sesame genotypes to applied irrigation treatments. The genotype Local Idleb recorded significantly the highest values of all investigated traits and the heighest seed yield (3.45 t. ha-1) as compared to other studied genotypes. Providing sesame crop with three irrigations surpassed in all investigated traits and recorded the highest seed yield (3.79 t.ha-1). It was recommended to provide sesame crop with three irrigations during critical stages (branches formation, capsules formation and seed filling).