The effect of sucrose on the chlorophyll content of healthy and vitrificated plants of some species of Gypsophylla panicolata micropropagated in vitro


Abstract in English

This study was conducted at the laboratories of Horticultural Department, Faculty of Agriculture, Damascus University to determine the effect of using three levels of sucrose (10, 30, 50 g /l) on chlorophyll content between healthy and vitrified plants of three varieties of Gypsophylla panicolata (Golantwo, Planshnet, Newlove). Results showed superiority of treatment 10 g / l sucrose in reducing vitrification percent to 36.78% compared with 62.57% for control (30 g/l) and with significant differences between varieties. Planshnet was the best variety in chlorophyll content either in healthy or in vitrified plantlets. Moreover, the treatment with sucrose of 10 g/l caused significant increment of chlorophyll content compared with other treatments. The high total chlorophyll content was 17.46 micrograms/ ml in healthy plants compared with 14.26 micrograms / ml in vitrification plants for Planshnet at 10 g/l sucrose.

References used

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Capellades, M., R. Lemeur and P. C. Debergh. 1991. Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell. Tiss and Org Cult, 25:19-20
Demeester, J. J., D. G. Matthÿs., B. Pascat and P. C. Debergh. 1995. Toward a controllable headspace composition. Growth, development, and headspace of a micropropagated Prunus rootstock in different containers. In Vitro Cell. Dev. Biol, 31:105-112

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