This study was conducted at the laboratories of Horticultural Department, Faculty of Agriculture, Damascus University to determine the effect of using three levels of sucrose (10, 30, 50 g /l) on chlorophyll content between healthy and vitrified plants of three varieties of Gypsophylla panicolata (Golantwo, Planshnet, Newlove). Results showed superiority of treatment 10 g / l sucrose in reducing vitrification percent to 36.78% compared with 62.57% for control (30 g/l) and with significant differences between varieties. Planshnet was the best variety in chlorophyll content either in healthy or in vitrified plantlets. Moreover, the treatment with sucrose of 10 g/l caused significant increment of chlorophyll content compared with other treatments. The high total chlorophyll content was 17.46 micrograms/ ml in healthy plants compared with 14.26 micrograms / ml in vitrification plants for Planshnet at 10 g/l sucrose.