Callus induction and plant regeneration from mature embryo in grain Sorghum (Sorghum bicolor.LMonech)
published by Damascus University
in 2013
in Field Crops
and research's language is
العربية
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Abstract in English
This Research was conducted in the laboratory of tissue culture affiliated to
the National Commission of Biotechnology (NCBT) during the period between
2011 -2012 for induction of callus from mature embryos for three local
Genotypes of grain Sorghum, regeneration of the plant from callus, rooting
plantlets and acclimatization in order to get a plant capable to grow in
greenhouse. The best concentration for sterilizing the plant and entering it in
use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4-
dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of
callus induction and embryogenic callus.
References used
Antonopoulou, G., H. Gavala, I. Skiadas, K. Angelopoulos, and G. Lyberatos. 2008. Biofuels generation from sweet sorghum: Fermenatative hydrogen production and anaerobic digestion of the remaining biomass. ioresour. echnol. 99:110-119
Bhaskaran, S. and R. H. Smith. 1990. Regeneration in cereal tissue culture: A review. Crop Sci., 30: 1328-1336
Bi, R., Kou, M. M., Chen, L. G., Mao, S .R and H. G. Wang. 2007. Plant regeneration through callus initiation from mature embryo of Triticum. Plant Breed. 126: 9-12