This current study was carried out on B41 grape rootstock micropropagated in vitro at the General Commission for Scientific Agricultural Research (GCSAR), Syria to study the effect of different concentrations of sodium chloride on the multiplication and rooting stages. The results showed that the highest average of survival plants (98%), the number of new buds (8.43) and plant length (8.58 cm) were obtained in the control treatment after 4 weeks from multiplication stage. The treatment with 50 mM of sodium chloride led to reduce the multiplication rates with significant differences, while the lowest plant multiplication had occurred with 100 mM of sodium chloride and no survival plants were remained by the treatment with 150 mM of sodium chloride during multiplication stage. The highest rooting rates (% 85.30), the number of roots (4.67) and root length (6.28 cm) were also obtained in the control treatment after 4 weeks from rooting stage. The treatment with 50 mM of sodium chloride led to reduce the rooting process with significant differences. The lowest rooting results were observed with 100 mM of sodium chloride and no rooting rate was observed when plants were treated with 150 mM of sodium chloride.