One hundred samples of Bacteriological swabs were obtained of medical team clothes, treatment and surgery tools, and patient covers and napkins, from treatment and surgery rooms in the hospital of maxillo-facial surgery at the Faculty of Dentistry - Damascus university, before utilization for checking the sterilization, and after utilization for detecting staphylococci and determining its species that spread in the course of different dental treatments and surgical procedures. In this study, sterility for all studied samples was shown before utilization, but after utilization we observed complete contamination in glove samples with percentage (100%), while the samples of masks and tools and napkins showed (50%) of contamination, and the samples of medical team clothes and patient covers kept most of them (80%) of their sterility after utilization.