This study has been done according to the immune –enzymology (ELISA) to find out the serological test of Delta hepatitis virus and serological test of B Viral hepatitis in 311 positive specimen of HBsAg. The Finding was as follows: 33 specimens (10.6 %) confirm the existence of anti – HD. As for HBV and HBD infection, six cases (18.18 %) of them were Coinfection and one case (3.03%) was severing coinfection and its early stage because of the existence of viral multiplication of the HBV (Ag HBe+). The rest of the cases 27 (18.81%) were Sur-infection among which two cases (6.06%) were accompanied by viral multiplication of Ag HBe+ of the HBV.