A field experiment was conducted at two environmentally different locations (Damascus and Dara'a), to evaluate the performance of 16 barley genotypes under rainfed conditions during the growing season of 2011-2012 in order to study in the response of some quantitative traits and determine the yield under drought conditions. The experiment was laid out according to the factorial complete randomized block design with three replicates for each genotype. Significant genetic variation was found in the response of barley genotypes to water stress. The performance of all genotypes was significantly superior in the less dry region (Izra’a-Daraa) compared to the more dry one (Abo jarash- Damascus) for all the examined parameters. Results revealed that the number of grains per spike was significantly higher in most lines compared to Arabi Abiad and Arabi Aswad, and the number of grains per spike was significantly higher in the line29 (43.17 grain per spike). The number of grains per m2 was significantly higher in line29,line64, Arabi Abiad and line14 (11177, 10809, 10802, 10744 grains per m2), respectively. The mean grain yield was significantly higher in the line64, line14 and Arabi Abiad. (479.3, 476 and 443 gram per m-2), respectively. Harvest index was significantly higher in all lines compared to Arabi Abiad and Arabi Aswad.