A known weight of Abrus seeds powder were soaked in cِhloroformmethanol (2:1) and extracted for 18 hours, the mixture then filtrated and reextracted with equal volume of water and evaporated to dryness. The crude extract is regarded as cِhloroform-methanol extract of Abrus Seeds (crab's eye). Chloroform-methanol extract was analyzed by HPLC and GC-MS chromatography for identification and purification of the extract components. Trigonelline and Abrine were isolated, a solution of a definite concentration was made from crab's eye extract and its pure components, trigonelline and Abrine, then oral administration of this solution of a definite concentration were given to the Alloxan diabetic rats.