The antimicrobial activity of aqueous extract of Zingiber Officinalis were tested for their antifungal activity against the following dermatophytes :Candida albicans, Cladosporium cladosporiosis , Cryptococcus neuphormans, Trichophyton violaceum and against other four bacteria : E. coli, Pseudomonas aeruginosa. Staphylococcus aureus, Streptococcus pyogenes. The extract used in traditional medicine for the treatment of nausea was tested in vitro through the Agar Disk Diffusion Method. The minimum inhibitory concentration (MIC) of extracts determined by the Agar dilution method ranged from 1.5 to 12.5 mcg. The most sensitive microorganisms to the extract were Candida albicans with MIC 1.5 and E coli with MIC 1.5 mcg.