Ninety-nine randomized samples of milk and white cheeses were collected from five districts in Damascus city during 2012-2013 to determinef Aflatoxin M1 using High Performance Liquid Chromatography (HPLC). The procedure for determination of Aflatoxin M1 was changed according to local circumnutates which gave sensitive and accurate results during 5.9 minute with r= 0.99. The average recovery was 83.88% for milk and 102.15% for cheeses samples, respectively.