Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus communis L.) grown in the field under natural conditions at Damascus countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected by 70% Ethanol and Clorox containing 5.25 % Sodium Hypoclorite with a drop of Tween 20 for different periods and concentrations before being placed onto MS basal medium. Established cultures were then transferred onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of 12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44 μM BA with 1.47 μM IBA and GA3 at 0.58 μM.