In this study, the effect of different concentrations of sodium chloride (NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some growth indicators of SO4 grape rootstock propagated in vitro was studied at the laboratories of General Commission for Scientific Agricultural Research (GCSAR), Damascus/Syria. Results indicated that the increase of NaCl concentration up to 100 mM in culture medium led to reduce the growth indicators (survival plants, average of the plant length and average number of buds) with significant differences when compared with the control plants after 4 weeks from culturing on multiplication media, while treatment with 150 mM of sodium chloride led to death of all plants. Using the concentrations 50 and 100 mM of NaCl also caused decreasing the total chlorophyll content in the leaves.